![]() ![]() Proteus morgani is less frequently associated with urinary tract infections than Proteus mirabilis- an explanation. Swarming and pathogenicity of Proteus mirabilis in the urinary tract. This work was supported by grants from the CNPq, CAPES, and FAPERGS. Four of them (citronellol, geraniol, α-terpineol, and terpinene-4-ol) are oxygenated compounds with hydroxyl groups, one is a cyclized monoterpenes alcohol (1,8-cineol), and three are monoterpene esters (citronellal, citral and pulegone). The eight compounds that effectively inhibit swarming were oxygenated monoterpenes. The reduction of the number of concentric rings and ring width are first indicatives of the interference of sub-inhibitory concentrations of terpenoids on swimming/swarming cell differentiation and swarming cell motility. Swarming inhibition was evidenced by a reduction on colony diameter, the number of concentric rings, and ring width ( Table 1 and Figure 1). These experiments showed that eight out of 17 compounds tested inhibited swarming significantly ( Table 1). mirabilis swarming behavior, LB swarming agar plates containing 1/10 MIC concentration of each compound were seeded with a drop (5 μL) of a stationary culture of wild-type L68 strain. mirabilis isolated from burn wounds with irradiance of 0.1watt/cm 2 in vitro, as evidenced by the effective reduction in the viability of bacteria at a dose of 120J/cm 2 corresponding to exposure time of 20 minutes.To evaluate the effect of the monoterpenes on P. As a conclusion, the blue laser irradiation at wavelength of 450 nm and 500mw had antibacterial effects on P. No significant reduction was recorded in CFU/ml at exposure time of 4 min (a dose of 24J/cm 2). a dose of 120J/cm 2, with irradiance of 0.1 watt/ cm 2. ![]() With the increase in laser dose (exposure times), the number of colonies and CFUs/ml were reduced, reaching a highest inhibition in CFU/ml at exposure time of 20 minutes, i.e. The results of antibiotic susceptibility test indicate that the entire isolates of P. mirabilis was irradiated by blue diode laser (450 nm, 500mw) and exposed to different doses (24, 48, 72, 96, 120J/cm 2) corresponding to respective exposure times (4, 8,12,16,20 minutes). A number of colonies and colony forming units (CFUs/ml) were achieved and correlated to controls. mirabilis was selected from other serial dilutions. mirabilis was mixed with normal saline solution. mirabilis was elected according to susceptibility to all antibiotics used. By using the disk diffusion method, susceptibility of the isolates to 12 different antibiotics was examined. The Bacteria were isolated and identified depending on their culture characteristics, biochemical tests, gram staining, and morphology, being finally confirmed by API 20E Test System. Seventy swab samples were collected from burn wounds of patients admitted to the burns unit in AL-Yarmouk teaching hospital in Baghdad, during the period from June to August 2019. ![]() The aim of this project is to study the influence of blue laser (450 nm) on the viability of the gram-negative bacteria Proteus mirabilis isolated from burn wounds, using different exposure times (i.e. The usage of blue laser has been considered as a therapeutic approach to prohibit the viability of bacterial species, but there is no agreement about optimum parameters to be used. ![]()
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